| 摘要: |
| 2021年11月~2022年11月,对洱海流域的藻类开展仪器和人工检测比对实验,通过分析比对数据,探讨和验证仪器的准确性、一致性及适用范围。结果表明:仪器检测在藻细胞密度和种属数上较人工偏高,但变化趋势总体一致,在一定程度上可以反映藻类的变化情况；仪器检测需调整藻细胞密度,最宜测定的藻细胞密度为1000~3000万个/L,仪器比对人工的相对偏差为±40%,仪器两次计数结果的相对偏差为±20%,适用于对数据精度要求不高的趋势性研究监测；仪器在样品浓缩5倍时的方法检出限为2.4×104个/L,空白实验验证的检出限高于方法检出限；含杂质或植物碎屑多的样品,仪器检测存在错误,不宜用仪器检测,应用人工检测,样品藻细胞密度低于500万个/L或高于10000万个/L,仪器检测偏高或偏低较多,误差相对较大,需进行处理后检测或采用人工检测。 |
| 关键词: 藻类 仪器检测 人工检测 比对 适用性 |
| DOI： |
| 分类号: |
| 基金项目:云南省财政项目 |
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| Comparison and Applicability of Alphacount2000 Algae Density Automatic Instrument with Manual Detection |
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yangpingping1,2,3, cunxiaodan1,2,3, lijin1,2,3, yangfenge1,2,3, huangsheng1,2,3
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1.Yunnan Provincial Ecological Environment Department'2.'3.s Ecological Environment Monitoring Station in Dali Prefecture
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| Abstract: |
| From November, 2021 to November, 2022, the algae in Erhai Lake Basin were compared by instruments and manual detection. By analyzing the comparison data, the accuracy, consistency and application scope of the instruments were discussed and verified. The results show that the cell density and species number of algae detected by instrument are higher than those detected by manual, but the change trend is generally consistent, which can reflect the change of algae to some extent. The density of algae cells needs to be adjusted for instrument detection. The most suitable density of algae cells is 10-30 million cells /L, the relative deviation of instrument comparison is 40%, and the relative deviation of instrument twice counting results is 20%, which is suitable for trend research and monitoring with low requirements on data accuracy. The method detection limit of the instrument is 2.4×104 pieces /L when the sample is concentrated 5 times, and the detection limit verified by blank experiment is higher than the method detection limit. For samples containing many impurities or plant debris, there are errors in instrument detection, so it is not suitable to use instrument detection. Manual detection is applied. The density of algae cells in samples is lower than 5 million /L or higher than 100 million /L, and the instrument detection is too high or too low, with relatively large errors, so it is necessary to carry out post-treatment detection or manual detection. |
| Key words: Algae instrument detection manual detection intercomparison applicability |
