| 摘要: |
| 毒嗅蓝藻会释放异味物质和毒素,影响水质,危害健康。传统显微镜蓝藻计数费时费力,更难以区分是否产毒产嗅,无法实现毒嗅蓝藻的精准监测。本研究基于多重荧光定量PCR(qPCR)技术,以16S,2-MIB和mcyE为靶向基因,构建基于多重PCR的毒嗅蓝藻检测方法,能够同时检测产毒蓝藻和产嗅蓝藻,检出限分别为：16S,101 copies/μl；2-MIB,101 copies/μl；mcyE,101 copies/μl,具有灵敏度高和稳定性强的特点。在太湖的应用显示,环境DNA多重定量PCR方法与传统形态学方法具有较高的一致性。该方法可用于连续性毒嗅蓝藻监测,提高效率,为蓝藻的快速监测与预警提供支撑。 |
| 关键词: qPCR 毒嗅蓝藻 环境DNA 2-MIB 蓝藻毒素 |
| DOI： |
| 分类号: |
| 基金项目:江苏省环境监测科研(1802)；“江苏省卓越博士后”资助项目(2022ZB811)。 |
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| Quantitative monitoring method for toxin-producing and olfactory-producing cyanobacteria based on environmental DNA |
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Rong Zheng,Lei Zhiming,Zhang Xiaowei
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1.Henan Provincial Environmental Monitoring Center,Henan;2.Nanjing E-Genomics Technology Co,Ltd,Nanjing;3.Nanjing University
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| Abstract: |
| Toxin-producing and odorous-producing cyanobacteria release odorous substances and cyanotoxins, impacting water quality and threatening human health. The traditional cyanobacteria monitoring method is time-consuming and laborious, and it cannot distinguish whether these cyanobacteria produce odorous substances or toxins. In this study, we have constructed a detection method for odorous-producing and toxin-producing cyanobacteria based on multiplex quantitative real-time PCR (qPCR) technology, targeting 16S, 2-MIB, and mcyE genes. It can simultaneously detect toxin-producing cyanobacteria and odor-producing cyanobacteria. The limits of detection were as follows: 16S, 101 copies/μl; 2-MIB, 101 copies/μl; mcyE 101 copies/μl. It is characterized by high sensitivity and strong stability. Furthermore, this advanced technique was used to investigate cyanobacteria in Taihu Lake, and the results showed that the multiplex quantitative PCR method shows a high level of consistency with traditional morphological methods. This approach can be used for continuous and efficient cyanobacteria monitoring and provide support for the rapid monitoring and early warning of potential cyanobacterial blooms. |
| Key words: qPCR toxin-producing and odorous-producing cyanobacteria environmental DNA 2-MIB, cyanotoxins |
