| 摘要: |
| 环境污染物,尤其是备受关注的新污染物,环境浓度低,复合污染评估难。转录组学作为开展早、较成熟的毒理基因组学技术,通过检测化学品暴露后细胞内所有基因表达的变化,在分子水平反映毒性效应大小和类型,具有检测时间早、检测指标多、受试物易得等优势,是传统毒性测试方法的有益补充。本研究选择江苏省某化工园区进行剂量依赖转录组学的生物毒性测试研究。研究表明,环境样品对人肝癌细胞的表观形态和存活率没有影响,未表现出急性毒性；但是转录组学测试数据显示各点位的综合毒性效应存在显著差异,污水处理厂排口的毒性效应相对较高,调控环境激素作用的基因表达改变,预示有内分泌干扰相关污染物的存在。该方法为识别和评估痕量新污染物的毒性效应和环境风险提供了新思路。 |
| 关键词: 生物毒性 细胞 剂量依赖转录组学 生物学通路 |
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| 基金项目:江苏省环境监测科研基金项目（2203）；江苏省卓越博士后资助项目（2022ZB811） |
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| Research and Application of Biotoxicity Testing Method based on Transcriptome in Chemical Industrial Park |
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| Abstract: |
| Environmental pollutants, especially high-concern emerging contaminants, have low environmental concentrations and are difficult to assess combined pollution. As an early and mature toxicogenomics technology, transcriptomics can detect the expression changes of all genes in cells after chemical exposure, and reflect the size and type of toxic effect at the molecular level. It has the advantages of early detection time, multiple detection indicators, and easy availability of test cells, which is a useful supplement to traditional toxicity testing methods. In this study, a wastewater treatment plant in a chemical park in Jiangsu province was selected to carry out the biotoxicity test by concertration-dependent transcriptome. The results showed that environmental samples had no effect on the morphology and survival rate of human hepatoma cells line, and did not show acute toxicity. However, the transcriptome data showed that there were significant differences in the combined toxic effects at each site. The toxic effects of the effluents from wastewater treatment plants were relatively high, and the differential expression of genes regulating the action of environmental hormones indicated the presence of endocrine disruption-related pollutants. This method provides a new insight for identifying and assessing the toxic effects and environmental risks of trace emerging contaminants. |
| Key words: Biotoxicity Cell Concertration-dependent transcriptome Biological pathway |
