|关键词: 氯霉素 液质联用 固相萃取
|Determination of Chloramphenicol in Water Samples by Solid Phase Extraction Ultra Performance Liquid Chromatography Electrospray Tandem Mass Spectrometry
YIN Yan min
|A rapid analytical method for chloramphenicol (CAP) in water samples was developed. Water samples were purified and concentrated by solid phase extraction (SPE) then detected by ultra performance liquid chromatographyelectrospray tandem massspectrometry (UPLC〖ＣＤ２〗MS/MS).The method used electrospray ionization, multiple reactions monitoring in negative ion mode to analyze CAP residue qualitatively and quantitatively. The analysis of CAP products can be finished in 5 minutes. The limit of determination of this method was 0.2ng/L. The average recovery rate of real samples was in the range of 76.2%～104%. This method was simple, sensitive and good repeatability.
|Key words: chloramphenicol UPLC-MS/MS SPE