引用本文:张皓, 张小琼,徐东炯,张翔.改良沉淀集卵法检测固体中蛔虫卵方法研究[J].环境监控与预警,2015,7(2):17-19
ZHANG Hao, ZHANG Xiao qiong, XU Dong jiong, ZHANG Xiang.Modified Sedimentation Method of Collecting Eggs for Detection of Ascaris Eggs in Various Solid Matrices[J].Environmental Monitoring and Forewarning,2015,7(2):17-19
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改良沉淀集卵法检测固体中蛔虫卵方法研究
张皓, 张小琼,徐东炯,张翔
江苏省环境保护水环境生物监测重点实验室,常州市环境监测中心,江苏 常州 213001
摘要:
采用改良后的沉淀集卵法对低(10个/100 g)、中(20个/100 g)、高(40个/100 g)3种不同浓度的加标样品进行蛔虫卵检测,比较其回收率。结果表明:不同浓度加标样品的回收率为高浓度>中浓度>低浓度。与相关文献实验结果比较表明,该方法不仅回收率较高、稳定性较好,且在检测低浓度样品时较漂浮集卵法具有一定优势。
关键词:  沉淀集卵法  蛔虫卵  加标样品  回收率
DOI:
分类号:R383.1+1
基金项目:环境标准制修订专项项目(1130)
Modified Sedimentation Method of Collecting Eggs for Detection of Ascaris Eggs in Various Solid Matrices
ZHANG Hao, ZHANG Xiao qiong, XU Dong jiong, ZHANG Xiang
Jiangsu Environmental Protection Key Laboratory for Aquatic Biomonitoring, Changzhou Environmental Monitoring Center, Changzhou, Jiangsu 213001,China
Abstract:
Modified sedimentation method was applied for detecting ascaris eggs in low (10 eggs per 100g), medium (20 eggs per 100g) and high (40 eggs per 100g) concentrations. Comparison of the recoveries at these three different spiked levels, high concentration ones had the highest recovery, followed by medium concentration ones, and low concentration ones.Compared with the experimental results in references,the recovery of this method was higher, and the stability was better. Also,this method was more appropriate for detecting samples in low concentration than flotation method.
Key words:  Sedimentation method of collecting eggs  Ascaris eggs  Spiked samples  Recovery