| 引用本文: | 徐超，张军毅*，朱冰川，宋挺，黄君，吴蔚.夏季太湖梅梁湾水体中细菌的群落结构[J].环境监控与预警,2015,7(1):37-40 |
| XU Chao,ZHANG Jun yi*,ZHU Bing chuan,SONG Ting,HUANG Jun,WU Wei.Extensive Profiling of a Bacterial Community during Summer in Meiliang Bay,Lake Taihu[J].Environmental Monitoring and Forewarning,2015,7(1):37-40 |
|
| 摘要: |
| 利用高通量测序技术，选择16S rRNA V6区作为目标片段，对夏季太湖梅梁湾水体中的细菌群落结构进行了分析，结果表明，共产生了101 427条优质序列，细菌为100 935条，占99.5%；在蓝藻暴发期间，共检测到14门，55属，610个操作分类单位。优势门类为蓝藻门(39.7%)，放线菌门(27.2%)和变形菌门(23.4%)，微囊藻属(21.0%)和聚球藻属(15.9%)为主要优势种。 |
| 关键词: 高通量测序 群落结构 16S rRNA 太湖 |
| DOI： |
| 分类号:X832 |
| 基金项目:江苏省环境监测科研基金资助项目（1320） |
|
| Extensive Profiling of a Bacterial Community during Summer in Meiliang Bay,Lake Taihu |
|
XU Chao,ZHANG Jun yi*,ZHU Bing chuan,SONG Ting,HUANG Jun,WU Wei
|
|
Wuxi Environmental Monitoring Central Station,Wuxi,Jiangsu 214121,China
|
| Abstract: |
| In order to explore the prokaryotic community during summer in Meiliang Bay,Lake Taihu, we performed high throughput sequencing of the 16S rRNA genes V6 hypervariable regions. The results showed that a total of 101 427 quality reads (bacterial reads 100 935, 99.5%) were obtained.Bacteria were found belonging to 14 phyla, 55 genera, and 610 OTUs. At the phylum level, the bacterial community composition was predominated by Cyanobacteria(39.7%), Actinobacteria(27.2%),Proteobacteria(23.4%).At the genus level, Microcystis(21.0%) and Synechococcus（15.9 %) was the most abundant genus. |
| Key words: High-throughput sequencing Community structure 16S rRNA Lake Taihu |
