引用本文:徐超,张军毅*,朱冰川,宋挺,黄君,吴蔚.夏季太湖梅梁湾水体中细菌的群落结构[J].环境监控与预警,2015,7(1):37-40
XU Chao,ZHANG Jun yi*,ZHU Bing chuan,SONG Ting,HUANG Jun,WU Wei.Extensive Profiling of a Bacterial Community during Summer in Meiliang Bay,Lake Taihu[J].Environmental Monitoring and Forewarning,2015,7(1):37-40
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夏季太湖梅梁湾水体中细菌的群落结构
徐超,张军毅*,朱冰川,宋挺,黄君,吴蔚
无锡市环境监测中心站, 江苏 无锡 214121
摘要:
利用高通量测序技术,选择16S rRNA V6区作为目标片段,对夏季太湖梅梁湾水体中的细菌群落结构进行了分析,结果表明,共产生了101 427条优质序列,细菌为100 935条,占99.5%;在蓝藻暴发期间,共检测到14门,55属,610个操作分类单位。优势门类为蓝藻门(39.7%),放线菌门(27.2%)和变形菌门(23.4%),微囊藻属(21.0%)和聚球藻属(15.9%)为主要优势种。
关键词:  高通量测序  群落结构  16S rRNA  太湖
DOI:
分类号:X832
基金项目:江苏省环境监测科研基金资助项目(1320)
Extensive Profiling of a Bacterial Community during Summer in Meiliang Bay,Lake Taihu
XU Chao,ZHANG Jun yi*,ZHU Bing chuan,SONG Ting,HUANG Jun,WU Wei
Wuxi Environmental Monitoring Central Station,Wuxi,Jiangsu 214121,China
Abstract:
In order to explore the prokaryotic community during summer in Meiliang Bay,Lake Taihu, we performed high throughput sequencing of the 16S rRNA genes V6 hypervariable regions. The results showed that a total of 101 427 quality reads (bacterial reads 100 935, 99.5%) were obtained.Bacteria were found belonging to 14 phyla, 55 genera, and 610 OTUs. At the phylum level, the bacterial community composition was predominated by Cyanobacteria(39.7%), Actinobacteria(27.2%),Proteobacteria(23.4%).At the genus level, Microcystis(21.0%) and Synechococcus(15.9 %) was the most abundant genus.
Key words:  High-throughput sequencing  Community structure  16S rRNA  Lake Taihu