引用本文:李旭冉,梅鹏蔚,王琳,姜伟.丙酮萃取分光光度法测定水中叶绿素a实验条件的优化[J].环境监控与预警,2016,8(3):25-27
LI Xu ran,MEI Peng wei,WANG Lin,JIANG Wei.Optimization of the Experimental Parameters in the Determination of Chlorophyll a in Water using Acetone Extraction Spectrophotometry[J].Environmental Monitoring and Forewarning,2016,8(3):25-27
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丙酮萃取分光光度法测定水中叶绿素a实验条件的优化
李旭冉,梅鹏蔚,王琳,姜伟
天津市环境监测中心,天津 300191
摘要:
基于丙酮萃取分光光度法,对叶绿素a测定方法进行深入研究,并通过单因素重复试验对冻存时间、冻存温度、萃取时间、萃取温度、细胞破碎方式、萃取剂浓度及萃取混合液提取方式等7方面的测定条件进行优化。优化后的实验条件为:水样经过抽滤后,置于-25℃低温冰箱中冻存12h后取出,在常温下加入分析纯丙酮,用力振摇2min至滤膜充分粉碎后定容到10mL,静置不超过0.5 h,在离心机内以4000r/min的速度离心,取上清液进行比色。
关键词:  叶绿素a  丙酮萃取  分光光度法
DOI:
分类号:O657.32
基金项目:
Optimization of the Experimental Parameters in the Determination of Chlorophyll a in Water using Acetone Extraction Spectrophotometry
LI Xuran,MEI Pengwei,WANG Lin,JIANG Wei
Tianjin Environmental Monitoring Center, Tianjin 300191, China
Abstract:
Based on acetone extraction spectrophotometry, the determination of chlorophyll a was investigated in depth. Seven experimental parameters were optimized through single factor repeat experiments, including freezing time, extraction time and temperature, cell lysis approaches, extractant concentrations and extraction approaches. The optimized experimental procedures were as follows. Water samples were filtered and stored at -25 ℃ for 12 h. The samples were then allowed to restore to room temperature before adding acetone and shaken vigorously for 2 min until the membrane was crushed completely. Samples were diluted to 10 mL and rested aside for less than 0.5 h. Finally, the samples were centrifuged at 4000 r/min and taken the supernatant for colorimetry.
Key words:  Chlorophyll a  Acetone extraction  Spectrophotometry