引用本文:王伟,卢志刚,张桂珍.DNPH衍生化采样-溶剂解析-高效液相色谱法同时测定木制品中25种醛酮化合物[J].环境监控与预警,2016,8(5):30-33
WANG Wei,LU Zhi gang,ZHANG Gui zhen.Simultaneous Determination of 25 Aldehydes and Ketones in Wood Materials by 2,4 Dinitrophenylhydrazine (DNPH) Derivatization Sampling and Solvent Desorption Coupled with High Performance Liquid Chromatography[J].Environmental Monitoring and Forewarning,2016,8(5):30-33
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DNPH衍生化采样-溶剂解析-高效液相色谱法同时测定木制品中25种醛酮化合物
王伟,卢志刚,张桂珍
作者单位
王伟1,卢志刚2*,张桂珍2 1. 南京市玄武区环境保护监测站江苏 南京 2100182. 江苏出入境检验检疫局江苏 南京 210001 
摘要:
建立了DNPH衍生化采样-溶剂解析-高效液相色谱测定木制品中醛酮化合物的方法。采样时需在2,4-二硝基苯肼吸附管前串联一只SEP-PAK脱臭氧小柱;采样体积应≤10 L,流量≤400 mL/min。该法对25种醛(酮)化合物对应的衍生物分离效果良好,在0.005~3 mg/L范围内线性良好,相关系数R2≥0.999 6,回收率为85.3%~113.5%,精密度为2.67%~4.56%。采样量为10 L时,25种醛(酮)化合物的检出限和定量限分别≤0.16和0.50μg/m3
关键词:  醛酮化合物  溶剂解析  衍生化  高效液相色谱  木制品
DOI:
分类号:O657.7+2
文献标识码:B
基金项目:江苏出入境检验检疫局计划项目(2010KJ26)
Simultaneous Determination of 25 Aldehydes and Ketones in Wood Materials by 2,4 Dinitrophenylhydrazine (DNPH) Derivatization Sampling and Solvent Desorption Coupled with High Performance Liquid Chromatography
WANG Wei,LU Zhi gang,ZHANG Gui zhen
Abstract:
The measurement of aldehydes and ketones in wood materials was established using 2,4 dinitrophenylhydrazine (DNPH) derivatization sampling and solvent desorption coupled with high performance liquid chromatography (HPLC). A SEP PAK column was attached to the front of the DNPH sampling column. Sample volume and flow speed were maintained below 10 L and 400 mL/min, respectively. The method showed good separation between 25 aldehydes and ketones, with good linearity in the range of 0.005~3mg/L. The correlation coefficient R2 was above 0.999 6, and the recovery rates and precision were in the range of 85.3%~113.5% and 2.67%~4.56%, respectively. When the sampling volume was 10 L, the detection limit and quantitative limit of 25 aldehydes and ketones were less than 0.16 and 0.50 μg/m3, respectively.
Key words:  Aldehyde and ketone  Solvent desorption  Derivatization  HPLC  Wood materials