引用本文:王伟,卢志刚,张桂珍.DNPH衍生化采样-溶剂解析-高效液相色谱法同时测定木制品中25种醛酮化合物[J].环境监控与预警,2016,8(5):30-33
WANG Wei,LU Zhi gang,ZHANG Gui zhen.Simultaneous Determination of 25 Aldehydes and Ketones in Wood Materials by 2,4 Dinitrophenylhydrazine (DNPH) Derivatization Sampling and Solvent Desorption Coupled with High Performance Liquid Chromatography[J].Environmental Monitoring and Forewarning,2016,8(5):30-33
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DNPH衍生化采样-溶剂解析-高效液相色谱法同时测定木制品中25种醛酮化合物
王伟,卢志刚,张桂珍1,2
1. 南京市玄武区环境保护监测站,江苏 南京 210018;2. 江苏出入境检验检疫局,江苏 南京 210001
摘要:
建立了DNPH衍生化采样-溶剂解析-高效液相色谱测定木制品中醛酮化合物的方法。采样时需在2,4-二硝基苯肼吸附管前串联一只SEP-PAK脱臭氧小柱;采样体积应≤10 L,流量≤400 mL/min。该法对25种醛(酮)化合物对应的衍生物分离效果良好,在0.005~3 mg/L范围内线性良好,相关系数R2≥0.999 6,回收率为85.3%~113.5%,精密度为2.67%~4.56%。采样量为10 L时,25种醛(酮)化合物的检出限和定量限分别≤0.16和0.50μg/m3
关键词:  醛酮化合物  溶剂解析  衍生化  高效液相色谱  木制品
DOI:
分类号:O657.7+2
文献标识码:B
基金项目:江苏出入境检验检疫局计划项目(2010KJ26)
Simultaneous Determination of 25 Aldehydes and Ketones in Wood Materials by 2,4 Dinitrophenylhydrazine (DNPH) Derivatization Sampling and Solvent Desorption Coupled with High Performance Liquid Chromatography
WANG Wei,LU Zhigang,ZHANG Guizhen1,2
1. Xuanwu District Environmental Monitoring Station, Nanjing, Jiangsu 210018, China;2. Jiangsu Entryexit Inspection and Quarantine Bureau, Nanjing, Jiangsu 210001, China
Abstract:
The measurement of aldehydes and ketones in wood materials was established using 2,4 dinitrophenylhydrazine (DNPH) derivatization sampling and solvent desorption coupled with high performance liquid chromatography (HPLC). A SEP PAK column was attached to the front of the DNPH sampling column. Sample volume and flow speed were maintained below 10 L and 400 mL/min, respectively. The method showed good separation between 25 aldehydes and ketones, with good linearity in the range of 0.005~3mg/L. The correlation coefficient R2 was above 0.999 6, and the recovery rates and precision were in the range of 85.3%~113.5% and 2.67%~4.56%, respectively. When the sampling volume was 10 L, the detection limit and quantitative limit of 25 aldehydes and ketones were less than 0.16 and 0.50 μg/m3, respectively.
Key words:  Aldehyde and ketone  Solvent desorption  Derivatization  HPLC  Wood materials