引用本文:张翔,张效伟,杨江华,周国栋.应用物种DNA条形码识别太湖流域部分底栖无脊椎动物种类[J].环境监控与预警,2016,8(6):18-21
ZHANG Xiang,ZHANG Xiao wei, YANG Jiang hua,ZHOU Guo dong.Applying DNA Barcoding for Identification of Some Invertebrate Macrozoobenthos at Taihu Lake Basin[J].Environmental Monitoring and Forewarning,2016,8(6):18-21
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应用物种DNA条形码识别太湖流域部分底栖无脊椎动物种类
张翔,张效伟,杨江华,周国栋1,2,3
1. 南京大学环境学院,江苏 南京 210000;2. 江苏省环境保护水环境生物监测重点实验室,江苏 常州 213001;3. 常州市环境监测中心,江苏 常州 213001
摘要:
将物种DNA条形码实际应用于太湖流域底栖无脊椎动物分类,并与形态学分类结果比对,结果表明,DNA条形码技术可应用于本流域底栖无脊椎动物分类,但现阶段无法替代形态学鉴定,主要原因是太湖流域底栖无脊椎动物的绝大多数物种COⅠ基因特征序列是未知的或是BLAST所拥有的分类程度不够,提出在今后的研究中应探索建立太湖流域底栖无脊椎动物COⅠ基因数据库。
关键词:  太湖流域  底栖无脊椎动物  DNA条形码  形态学分类
DOI:
分类号:X835
文献标识码:B
基金项目:国家水体污染控制与治理科技重大专项基金资助项目(2012ZX07506-003)
Applying DNA Barcoding for Identification of Some Invertebrate Macrozoobenthos at Taihu Lake Basin
ZHANG Xiang,ZHANG Xiaowei, YANG Jianghua,ZHOU Guodong1,2,3
1. School of Environment, Nanjing University, Nanjing, Jiangsu 210000, China;2. Jiangsu Environmental Protection Key Laboratory for Aquatic Biomonitoring, Changzhou, Jiangsu 213001, China;3. Changzhou Environmental Monitoring Center, Changzhou, Jiangsu 213001, China
Abstract:
DNA barcoding was applied for identification of invertebrate macrozoobenthos at Taihu Lake basin and the results were compared with those obtained from morphological classification. The results demonstrated that DNA barcoding could be applied to classify invertebrate macrozoobenthos at the basin, but at this stage could not replace morphological classification. The main reason was that there were unknown COI characteristic gene sequences for most of the invertebrate macrozoobenthos species or that the degree of classification was not enough for BLAST. It was proposed to explore and establish the gene data bank for invertebrate macrozoobenthos at Taihu Lake basin in future studies.
Key words:  Taihu Lake basin  Invertebrate macrozoobenthos  DNA barcoding  Morphological classification