| 摘要: |
| 建立了固相萃取-高效液相色谱-质谱联用法对雌三醇、氟哌噻吨、炔雌醇、雄烯二酮、雌二醇、雌酚酮、睾酮共7种类固醇激素的检测方法。样品经C18萃取小柱富集净化，采用内标法定量时，方法的检出限为 0.66~1.95 ng/L，R＞0.998，回收率为78.5%~94.6%，相对标准偏差为1.1%~7.7%。采用该方法对上海市地表水进行测定，在2个采样点中检出雌酚酮（1.88 ng/L）和雄烯二酮（1.48 ng/L）。 |
| 关键词: 固相萃取 高效液相色谱-质谱 类固醇激素 地表水 |
| DOI： |
| 分类号:X832；0657.63 |
| 文献标识码:B |
| 基金项目:上海市环保科研基金资助项目（2015-82） |
|
| Determination of Steroid Hormones in Surface Water by LC-MS/MS |
|
SUN Zhou lin
|
|
Shanghai Environmental Monitoring Center, Shanghai 200235, China
|
| Abstract: |
| A Solid-Phase Extraction and High-Performance Liquid Chromatography-Mass Spectrometry technique was developed to simultaneously detect seven steroid hormones (Estriol, Equilin, 17α-Ethynylestradiol, 4-Androstenedione, 17β-Estradiol, Estrone, Testosterone). The samples were purified and enriched by the SPE-C18 column. The internal standard method was adopted to quantify,the detection limit was between 0.66~1.95 ng/L,R＞0.998,recoveries was between 78.5%~94.6%,RSD was between 1.1%~7.7%. The proposed method was applied to analyze surface water samples in Shanghai. The trace concentrations were observed for Estrone (1.88 ng/L) and 4-Androstenedione (1.48 ng/L) in two sampling points. |
| Key words: Solid Phase Extraction Liquid Chromatography Mass Spectrometry Steroid hormone Surface water |
