引用本文:孙琳.固相萃取-高效液相色谱-质谱联用法用于地表水中类固醇激素测定[J].环境监控与预警,2018,10(5):26-29
SUN Zhou lin.Determination of Steroid Hormones in Surface Water by LC-MS/MS[J].Environmental Monitoring and Forewarning,2018,10(5):26-29
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固相萃取-高效液相色谱-质谱联用法用于地表水中类固醇激素测定
孙琳
上海市环境监测中心,上海 200235
摘要:
建立了固相萃取-高效液相色谱-质谱联用法对雌三醇、氟哌噻吨、炔雌醇、雄烯二酮、雌二醇、雌酚酮、睾酮共7种类固醇激素的检测方法。样品经C18萃取小柱富集净化,采用内标法定量时,方法的检出限为 0.66~1.95 ng/L,R>0.998,回收率为78.5%~94.6%,相对标准偏差为1.1%~7.7%。采用该方法对上海市地表水进行测定,在2个采样点中检出雌酚酮(1.88 ng/L)和雄烯二酮(1.48 ng/L)。
关键词:  固相萃取  高效液相色谱-质谱  类固醇激素  地表水
DOI:
分类号:X832;0657.63
文献标识码:B
基金项目:上海市环保科研基金资助项目(2015-82)
Determination of Steroid Hormones in Surface Water by LC-MS/MS
SUN Zhou lin
Shanghai Environmental Monitoring Center, Shanghai 200235, China
Abstract:
A Solid-Phase Extraction and High-Performance Liquid Chromatography-Mass Spectrometry technique was developed to simultaneously detect seven steroid hormones (Estriol, Equilin, 17α-Ethynylestradiol, 4-Androstenedione, 17β-Estradiol, Estrone, Testosterone). The samples were purified and enriched by the SPE-C18 column. The internal standard method was adopted to quantify,the detection limit was between 0.66~1.95 ng/L,R>0.998,recoveries was between 78.5%~94.6%,RSD was between 1.1%~7.7%. The proposed method was applied to analyze surface water samples in Shanghai. The trace concentrations were observed for Estrone (1.88 ng/L) and 4-Androstenedione (1.48 ng/L) in two sampling points.
Key words:  Solid Phase Extraction  Liquid Chromatography Mass Spectrometry  Steroid hormone  Surface water