引用本文:曹军,陈鑫琪,张文龙.长江南京段入江支流底质多营养级微生物多样性研究[J].环境监控与预警,2020,12(6):41-46
CAO Jun, CHEN Xin-qi, ZHANG Wen-long.Multi Trophic Multi Trophic Microbial Diversity in the Tributaries Sediment of Nanjing Section of the Yangtze River[J].Environmental Monitoring and Forewarning,2020,12(6):41-46
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长江南京段入江支流底质多营养级微生物多样性研究
曹军,陈鑫琪,张文龙
作者单位
曹军1,陈鑫琪2,张文龙3 1.江苏省环境监测中心江苏 南京 2100192.江苏省南京环境监测中心江苏 南京 2100133.河海大学江苏 南京 210098 
摘要:
基于16S rRNA和18S rRNA高通量测序技术,研究了长江南京段21条主要入江支流不同水期底质多营养级微生物的群落结构特征,利用Shannon指数等分析了样品中微生物群落的多样性。结果表明,42个样品的细菌群落归属于285个纲水平上的OTUs (operational taxonomic units),归属于62个门;优势菌门为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)等,优势菌纲为β-变形菌纲、α-变形菌纲、δ-变形菌纲等。原生动物中的优势类群为不等鞭毛类(Heterknta)、囊泡虫类(Alveolata)和绿色植物(Chloroplastida),后生动物中的优势类群为节肢动物(Arthropoda)、轮虫(Rotifera)和线虫(Nematoda)。平水期底质的细菌、原生及后生动物的多样性相对较低,入江支流的水生态系统相对不稳定,尤其是金川河、七里河等。
关键词:  多营养级  微生物群落  多样性  入江支流  长江南京段
DOI:
分类号:X826
文献标识码:B
基金项目:江苏省环境监测科研基金资助项目(1914)
Multi Trophic Multi Trophic Microbial Diversity in the Tributaries Sediment of Nanjing Section of the Yangtze River
CAO Jun, CHEN Xin-qi, ZHANG Wen-long
Abstract:
Based on 16S rRNA and 18S rRNA high throughput sequencing technology, the community structure characteristics of multi trophic level microbes in 21 main tributaries in Nanjing section of the Yangtze River were analyzed. The Shannon index was used to analyze the diversity of microbial communities in the samples. The results showed that the bacterial community of 42 samples belonged to the OTUs (operational taxonomic units) of 285 classes, 62 phyla; the dominant phyla were Proteobacteria, Acidobacteria and Chloroflexi, etc., and the dominant classes were β Proteobacteria, αProteobacteria and δ Proteobacteria, etc. The dominant groups of protozoa were Heterknta, Alveolata and Chloroplastida, while the dominant groups of Metazoa were Arthropoda, Rotifera and Nematoda. The diversity of bacteria, protozoa and metazoa in the sediment is relatively low in the normal water period, which indicates that the water ecosystem in the tributaries is relatively unstable, especially in Jinchuan River and Qili River.
Key words:  Multi trophic level  Microbial community  Diversity  Tributaries of the Yangtze River  Nanjing section of the Yangtze River