|关键词: 碳纳米管 毒性作用 酶 抗氧化系统 锦鲫
|Tissue Damage and Liver Oxidative Damage of Goldfish (Carassius Auratus) Induced by Two Typical Low Concentration Carbon Nanotubes Exposure
KAN Hai-feng，LU Ming-xia，HUANG Ji-tao， GAO Shi-xiang
1.Fujian Provincial Key Laboratory of Featured Materials in Biochemical Industry, Fujian Province University Key Laboratory of Green Energy and Environment Catalysis,et ai
|The impacts of SWCNT and MWCNT OH on the antioxidant defense system of juvenile goldfish (Carassius auratus) were studied by semi static exposure method. SWCNT and MWCNT OH were found to induce histopathological and oxidative damages in liver. The results showed that the antioxidant enzymes SOD and CAT activity were inhibited significantly in the liver of goldfish exposed to 10, 100 and 1 000 μg/L individually for 28 d. Individual exposure to SWCNT or MWCNT OH at 14 d resulted in histopathological gill changes as follows: aneurisms, oedema in the secondary lamellae and hyperplasia in the primary lamellae. SWCNT and MWCNT OH exposure was also found to induce necrosis and vacuolization in liver at the high dose of 1000 μg/L. However, no changes were observed in liver at the low SWCNT or MWCNT OH exposure level (dose of 10 μg/L) compared to control. No lethal effects were observed during the test. With the increase of exposure days, two carbon nanotubes could inhibit SOD and CAT enzyme, and the inhibition was obvious in the group with high concentration, and the antioxidant system of the liver was damaged. The results indicated that the long term impacts of carbon nanotubes to fish need to be further investigated.
|Key words: Carbon Nanotubes Toxic effects Enzymes Oxidative stress Goldfish (Carassius Auratus)