| 引用本文: | 沈燕，贾舒宇，李紫涵，张徐祥，任洪强.水环境中致病菌分子生物学检测技术研究进展[J].环境监控与预警,2020,12(5):1-13 |
| SHEN Yan, JIA Shu-yu, LI Zi-han, ZHANG Xu-xiang, REN Hong-qiang.A Review on Molecular Biologicaltechnologies for Detection of Pathogenic Bacteria in Water Environments[J].Environmental Monitoring and Forewarning,2020,12(5):1-13 |
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| 摘要: |
| 总结了水环境中主要致病菌及其导致的疾病；介绍了检测水环境样品中致病菌的聚合酶链式反应（PCR）、实时定量PCR、等温扩增技术、生物传感器和高通量测序等分子生物学技术研究进展；分析了不同检测技术的优缺点和应用特点；提出了水环境中致病菌分子生物学检测技术的未来发展方向，为水环境中致病菌分析检测和风险控制提供研究思路和技术支撑。 |
| 关键词: 致病菌 水环境 检测方法 分子生物技术 |
| DOI： |
| 分类号:X832；X172 |
| 文献标识码:A |
| 基金项目:国家自然科学基金青年基金资助项目（51908274）；中国博士后科学基金资助面上项目（2018M640475），南京大学卓越研究计划资助项目（ZYJH005） |
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| A Review on Molecular Biologicaltechnologies for Detection of Pathogenic Bacteria in Water Environments |
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SHEN Yan, JIA Shu-yu, LI Zi-han, ZHANG Xu-xiang, REN Hong-qiang
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School of Environment, Nanjing University, Nanjing, Jiangsu 210093, China
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| Abstract: |
| This study summarizes the main pathogenic bacteria and corresponding diseases in water environment, and the latest development of the molecular biological techniques of aquatic pathogens including polymerase chain reaction (PCR), quantitative real time PCR, isothermal amplification technique, biosensors, and high throughput sequencing. The advantages, disadvantages, and application characteristics of these different detection techniques are also analyzed. Furthermore, this study suggests the future development direction of molecular biological technologies for detecting pathogenic bacteria in water environment and tries to provide new research ideas and technological supports for detection and control of aquatic pathogens. |
| Key words: Pathogenic bacteria Water environment Detection technology Molecular biotechnology |
