引用本文:高雅倩,于凡芮,李蕾,陈建秋,刘艳华,郭瑞昕.水体中黑碳对斑马鱼胚胎的生长发育危害识别[J].环境监控与预警,2022,14(5):160-166
GAO Ya-qian, YU Fan-rui, LI Lei, CHEN Jian-qiu, LIU Yan-hua, GUO Rui-xin.Hazard Identification of Black Carbon on the Growth and Development of Zebrafish Embryos in Aquatic Environment[J].Environmental Monitoring and Forewarning,2022,14(5):160-166
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水体中黑碳对斑马鱼胚胎的生长发育危害识别
高雅倩,于凡芮,李蕾,陈建秋,刘艳华,郭瑞昕
中国药科大学工学院,江苏 南京 211198
摘要:
黑碳(BC)是含碳物质不完全燃烧后产生的残留物,广泛分布于水体、土壤和大气中,目前对于BC对水生生物的生态效应研究还十分有限。通过燃烧煤油获得BC,以斑马鱼胚胎为模式生物,研究了质量浓度分别为0,0.05,0.5和5 mg/L的BC悬浮溶液对斑马鱼早期生命阶段的生长发育毒性。结果表明,煤油源BC对斑马鱼具有明显的毒性作用,与对照组相比,BC暴露明显增加了斑马鱼的死亡率和心率,显著降低了孵化率和体长,导致斑马鱼形态畸形并且畸形率呈浓度依赖性。5mg/L的BC对斑马鱼的各项生长发育指标影响并非是最大的,可能与BC颗粒在水溶液中的聚集有关。该研究为BC在水生环境中的危害识别提供实验依据。
关键词:  黑碳  斑马鱼胚胎  发育毒性  致畸作用  危害识别
DOI:
分类号:X835
基金项目:中国药科大学创新创业培养基金资助项目(202210316357);国家级大学生创新创业训练计划支持联合基金资助项目(202210316006Z)
Hazard Identification of Black Carbon on the Growth and Development of Zebrafish Embryos in Aquatic Environment
GAO Ya-qian, YU Fan-rui, LI Lei, CHEN Jian-qiu, LIU Yan-hua, GUO Rui-xin
Faculty of Engineering, China Pharmaceutical University, Nanjing,Jiangsu 211198,China
Abstract:
Black carbon (BC) is formed by the incomplete combustion of carbonaceous substances, and is widely distributed in water, soil and atmosphere. However, studies on the ecological effects of BC on aquatic organisms are still limited. This study aimed to evaluate the impact of BC on zebrafish embryo and larvae to reveal the growth and developmental toxicity of the BC in the early life stage of model aquatic organism. The BC used in this study was produced by kerosene combustion and the concentration of the BC in testing groups was set as 0, 0.05, 0.5 and 5 mg/L, respectively. Our results showed the significant impact of BC on zebrafish embryos. Compared with the control, the mortality and heart rate of the embryos increased significantly after the BC exposure and the BC could also reduce the hatching rate and the body length of the larvae. Morphological deformity also occurred and was concentration dependent. However, the most serious impact of the BC was not obtained in the highest concentration group (5 mg/L), which may be related to the aggregation of BC particles in aqueous solution. This study provided the experimental basis for hazard identification of BC in the aquatic environments.
Key words:  Black carbon  Zebrafish embryo  Developmental toxicity  Teratogenic effect  Hazard identification.