引用本文:卢勇杰,孙巍,李嘉敬,陈心如,邝春议,朱颖,夏春雨.淡水中氨氮高效快速测定方法的优化研究[J].环境监控与预警,2023,15(2):56-61
LU Yong-jie, SUN Wei, LI Jia-jing, CHEN Xin-ru, KUANG Chun-yi, ZHU Ying, XIA Chun-yu.Optimization of an Efficient and Rapid Method for the Determination of Ammonia Nitrogen in Fresh Water[J].Environmental Monitoring and Forewarning,2023,15(2):56-61
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淡水中氨氮高效快速测定方法的优化研究
卢勇杰,孙巍,李嘉敬,陈心如,邝春议,朱颖,夏春雨
广东石油化工学院,生物与食品工程学院,广东 茂名 525000
摘要:
基于氨氮与纳氏试剂进行显色反应的原理,建立反应体系为1 mL的小体系淡水中氨氮的测定方法。新方法的显色剂用量为20μL、显色时间为10~30 min、盐度<0.5%、pH值为3~11, 纳氏试剂以6 000 r/min、5 min进行离心处理,采用酶标仪96孔板在420 nm波长下测定显色反应溶液的吸光度。将该方法与《水质 氨氮的测定 纳氏试剂分光光度法》(HJ 535—2009)(国标法)测定氨氮的吸光度进行正交验证,结果表明2种方法具有良好的拟合度。新方法的检测范围从国标法的0~2.0 mg/L提升到0~4.8 mg/L。方法测定淡水中氨氮的质量浓度具有简便、连续、快速、高批量的优点,适用于实地、实时地测定淡水中的氨氮。
关键词:  淡水  氨氮  小体系  纳氏试剂分光光度法  酶标仪
DOI:
分类号:X832
基金项目:国家自然科学青年基金项目(42006137);广东省基础与应用基础研究基金项目(2022A1515010539);广东石油化工学院科研基金人才引进项目(2019rc108);高位池海水养虾尾水无害化处理示范项目;广东省重点领域研发计划项目(2018B020205002);广东石油化工学院2021年度教育教学改革研究项目(2021JY29);大学生创新训练项目(73321101,73322049,73322094)
Optimization of an Efficient and Rapid Method for the Determination of Ammonia Nitrogen in Fresh Water
LU Yong-jie, SUN Wei, LI Jia-jing, CHEN Xin-ru, KUANG Chun-yi, ZHU Ying, XIA Chun-yu
School of Biotechnology and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China
Abstract:
Based on the principle of color reaction between ammonia nitrogen and Nessler’s reagent, a method for the determination of ammonia nitrogen in fresh water was established with a reaction system of 1 mL. The dosage of developer was 20 μL, the color development time was 10~30 min, salinity was less than 0.5%, pH value was 3~11, and Nessler’s reagent was centrifuged at 6 000 r/min for 5 min in this method. The absorbance of the color reaction solution was measured at 420 nm by using the 96 well plate of the enzyme marker. The orthogonal validation was carried out between the absorbance of the new method and the absorbance of ammonia nitrogen determined in Water Quality Determination of Ammonia Nitrogen Nesslers Reagent Spectrophotometry (HJ 535—2009) (national standard method). The result indicated that the new method showed a good fit with the national standard method. Moreover, the detection range was increased from 0~2.0 mg/L in the national standard method to 0~4.8 mg/L in the new method. The new method for the determination of ammonia nitrogen in fresh water showed the advantages of simplicity, continuity, rapidity and high volume, which was suitable for the field and real time determination of ammonia nitrogen in fresh water.
Key words:  Fresh water  Ammonia nitrogen determination  Small system  Nessler reagent spectrophotometry  Enzyme labeling instrument