| 摘要: |
| 合成了1种基于香豆素和嘌呤衍生物的高效且易于制备的荧光探针CMP，并对该探针检测土壤中的钴离子（Co2+）相关性能进行测试。实验结果表明，探针CMP在4-羟乙基哌嗪乙磺酸（HEPES）缓冲溶液（pH 值=7.4）中对Co2+表现出高灵敏度、高选择性和快速响应（＜1min），且一旦与Co2+结合后，可以观察到探针CMP溶液的颜色从黄色变为粉红色。探针CMP检测Co2+的最佳pH值为7.0~11.0。通过荧光滴定实验，计算出探针CMP的检出限为0.26 mg/kg。对土壤和沉积物标准样品进行测定，测定结果均在标准值范围内，相对标准偏差（RSD）为1.3%~2.7%，具有较高的准确度和精密度。该方法具有高选择性和高灵敏度，适用于高效、快速、准确地检测土壤中Co2+。 |
| 关键词: 探针CMP 香豆素 嘌呤衍生物 钴离子 席夫碱 |
| DOI：10.3969/j.issn.1674-6732.2023.06.008 |
| 分类号:X833 |
| 基金项目:江苏省环境监测科研基金项目（1907） |
|
| Synthesis of A Purine Fluorescent Probe and Its Performance in Co2+ Detection |
|
DONG Zheng1， WANG Chengxi1， WANG Lin1， LU Hongfei2
|
|
1.Jiangsu Zhenjiang Environmental monitoring Center, Jiangsu Province Zhenjiang Soil Characteristic Laboratory， Zhenjiang, Jiangsu 212000,China; 2. Jiangsu University of Science and Technology, Zhenjiang, Jiangsu 212003, China
|
| Abstract: |
| A high efficient and easy to be prepared fluorescent probe CMP based on coumarin and purine derivatives was synthesized， and its performance in detecting cobalt ions (Co2+) in soil was tested. The experimental results showed that the probe CMP exhibited high sensitivity， selectivity， and rapid response (<1 min) to Co2+ in a 4 hydroxyethyl piperazine ethanesulfonic acid (HEPES) buffer solution (pH=7.4)， and once combined with Co2+， the color of the probe CMP solution could be observed to change from yellow to pink. The optimal pH value for detecting Co2+ with probe CMP is between 7.0 and 11.0. The fluorescence titration experiments showed the detection limit of the probe CMP was 0.26 mg/kg. The determination of soil and sediment standard samples was carried out， and the results were within the standard value range， with a relative standard deviation (RSD) of 1.3%～2.7%， indicating high accuracy and precision. This method has high selectivity and sensitivity， and is suitable for efficient， fast， and accurate detection of Co2+ in soil. |
| Key words: Probe CMP Coumarin Purine derivatives Cobalt ion Schiff base |
