| 引用本文: | 杨一艳,李泽坤,杨萍萍,寸晓丹,李金,杨凤娥,黄升.AlphaCount 2000藻密度自动检测仪与人工比对及适用性探究[J].环境监控与预警,2025,17(1):67-73 |
| YANG Yiyan,LI Zekun,YANG Pingping,CUN Xiaodan,LI Jin,YANG Fenge,HUANG Sheng.Comparative Analysis and Applicability Exploration of AlphaCount 2000 Algae Density Detector Versus Manual Detection[J].Environmental Monitoring and Forewarning,2025,17(1):67-73 |
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| 摘要: |
| 于2021年11月—2022年11月，对洱海流域的藻类开展仪器检测和人工检测比对实验，通过分析比对数据，探讨和验证了仪器的准确性、一致性及适用范围。结果表明，仪器检测在藻细胞密度和种属数上较人工检测偏高，但变化趋势总体一致，在一定程度上可以反映藻类的变化情况；仪器检测须调整藻细胞密度，最宜测定的藻细胞密度为1 000～3 000万个/L；仪器检测与人工检测的相对偏差为±40%，仪器2次计数结果的相对偏差为±20%，适用于对数据精度要求不高的趋势性研究监测；仪器在样品浓缩5倍时的方法检出限为2.4×104 个/L，空白实验验证的检出限高于方法检出限；含杂质或植物碎屑多的样品，仪器检测容易产生错误结果，因此应采用人工检测；样品藻细胞密度＜500万个/L或＞10 000万个/L时，仪器检测偏高或偏低较多，误差相对较大，须进行稀释处理后再检测或进行人工检测。 |
| 关键词: 藻类 仪器检测 人工检测 比对 适用性 |
| DOI：DOI：10.3969/j.issn.1674-6732.2025.01.011 |
| 分类号:X835 |
| 基金项目:云南省财政项目（530000210000000016975） |
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| Comparative Analysis and Applicability Exploration of AlphaCount 2000 Algae Density Detector Versus Manual Detection |
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YANG Yiyan1， LI Zekun1*， YANG Pingping1， CUN Xiaodan1， LI Jin1， YANG Fenge1， HUANG Sheng2
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1. Dali Ecological and Environmental Monitoring Station of Yunnan Provincial Department of Ecology and Environment， Dali， Yunnan 671000， China； 2. Hangzhou Green Clean Environmental Technology Company Limited， Hangzhou， Zhejiang 311100，China
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| Abstract: |
| From November 2021 to November 2022， the algae in the Erhai Lake Basin were compared by instruments and manual detection. By analyzing the comparison data， the accuracy， consistency and application scope of the instruments were discussed and verified. The results show that the cell density and species number of algae detected by the instrument are higher than those detected manually， but the changing trend is generally consistent， which can reflect the change of algae to some extent. The density of algae cells needs to be adjusted for instrument detection. The most suitable density of algae cells is 10~30 million cells/L， the relative deviation of instrument comparison is ± 40%， and the relative deviation of instrument twice counting results is ± 20%， which is suitable for trend research and monitoring with low requirements on data accuracy. The method detection limit of the instrument is 2.4×104 cells/L when the sample is concentrated 5 times， and the detection limit verified by the blank experiment is higher than the method detection limit. For samples containing many impurities or plant debris， instrument detection yields many errors， making manual detection more suitable. When the density of algae cells in samples is lower than 5 million /L or higher than 100 million /L， the instrument detection can be too high or too low， with relatively large errors. In such cases， it is necessary to perform detection after concentration or dilution treatment or conduct manual detection instead. |
| Key words: Algae Instrument detection Manual detection Intercomparison Applicability |
