| 摘要: |
| 建立了超声提取-高效液相色谱法测定土壤中氨基苯磺酸的方法。以甲醇/水(V∶V=1∶1)为萃取溶剂，室温下进行超声波提取，通过固相萃取小柱净化后，以带季铵基团的聚乙烯醇离子色谱柱分离，二极管阵列检测器测定。该实验方法具备较好的抗干扰性，方法曲线相关系数r＞0.999，取样量为5 g时，对、间、邻氨基苯磺酸的方法检出限分别为0.1，0.2和0.2 mg/kg，加标回收率分别为83.5%~99.5%，83.1%~98.0%和77.8%～92.5%。对5个地区的土壤样品加标回收实验结果表明，该方法适用性较强，可满足实际样品的测定。 |
| 关键词: 高效液相色谱 土壤 对氨基苯磺酸 间氨基苯磺酸 邻氨基苯磺酸 |
| DOI： |
| 分类号:X833；O657.7+2 |
| 文献标识码:B |
| 基金项目: |
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| Study on Ultrasonic Extraction High Performance Liquid Chromatography Method for Determination of Aminobenzene Sulfonic Acids in Soil |
|
LI Guang-ming
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CCIC Physical and Chemical Testing Co. Ltd.，Shanghai 200436，China
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| Abstract: |
| An Ultrasonic Extraction High Performance Liquid Chromatography method for the determination of aminobenzene sulfonic acids in soil sample was established. Using methanol water(V∶V=1∶1) as extraction solvent, ultrasonic extraction was performed at room temperature, sample extract was cleaned up by solid phase extraction cartridge, separated by a polyvinyl alcohol ion chromatographic column bonded with quaternary ammonium group, and analyzed by diode array detector. The method showed good anti interference performance, the linear correlation coefficient r＞0.999, and the method detection limit of ρAminobenzene sulfonic acid was 0.1 mg/kg, m Aminobenzene sulfonic acid was 0.2 mg/kg while o Aminobenzene sulfonic acid was 0.2 mg/kg when sample weight was 5 g. The recoveries were 83.5%~99.5%, 83.1%~98.0% and 77.8%~92.5%, respectively. The results of the recovery experiment of soil samples in 5 regions showed that the method has strong applicability and can meet actual needs. |
| Key words: HPLC Soil ρAminobenzene sulfonic acid m Aminobenzene sulfonic acid o Aminobenzene sulfonic acid |
