| 摘要: |
| 利用加速溶剂萃取（ASE）、固相微萃取（SPME）和气相色谱-串联质谱法建立了一种适用于鱼肉中14种硝基多环芳烃（NPAHs）的检测方法。优化了ASE吸附剂种类，SPME萃取时间和萃取温度等条件。结果表明，最佳的吸附剂为中性氧化铝，萃取时间为35 min，萃取温度为55℃。在最优条件下，该方法的检测限为0.03～0.7 ng/g，加标回收率为59.1%～106%，相对标准偏差为1.7%～12%。该方法在准确度、精密度和检测限方面能够满足鱼肉样品中14种NPAHs实际检测的要求。 |
| 关键词: 加速溶剂萃取 固相微萃取 气相色谱-串联质谱法 鱼肉 硝基多环芳烃 |
| DOI：DOI：10.3969/j.issn.1674-6732.2025.01.009 |
| 分类号:X835 |
| 基金项目:国家自然科学基金资助项目（42307469）；2023年江苏省研究生科研与实践创新项目（1783） |
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| Determination of Fourteen Kinds of Nitro-Polycyclic Aromatic Hydrocarbons in Fish Meat by Accelerated Solvent Extraction-Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry |
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FANG Zhihong， ZHANG Xinqing， YAN Sirui， KONG Jijie*， HE Huan
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School of Environment， Nanjing Normal University， Nanjing， Jiangsu 210023， China
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| Abstract: |
| A method using accelerated solvent extraction（ASE）， solid phase microextraction（SPME）， and gas chromato-graphy-mass spectrometry was established for the determination of 14 nitro-polycyclic aromatic hydrocarbons（NPAHs） in fish samples. The conditions of ASE adsorbent type， extraction time， and extraction temperature were optimized. The results showed that the best adsorbent was neutral alumina， the extraction time was 35 min， and the extraction temperature was 55 ℃. Under optimal conditions， the detection limits ranged from 0.03 to 0.7 ng/g， with spike recoveries ranging from 59.1% to 106% and relative standard deviation ranging from 1.7% to 12%. The accuracy， precision， and detection limit of 14 NPAHs in fish meat could meet the requirements of actual sample analysis. |
| Key words: Solid phase microextraction Accelerated solvent extraction GC-MS Fish meat Nitro-polycyclic aromatic hydrocarbons |
