| 引用本文: | 顾杰，郭敏，吉贵祥，石利利.利用转基因斑马鱼探究四溴联苯醚（BDE-47）的神经毒性作用[J].环境监控与预警,2020,12(5):102-107 |
| GU Jie, GUO Min, JI Gui-xiang, SHI Li-li.Detecting the Neurotoxicity Effects of 2,2’,4,4’Tetrabromodiphenyl Ether Using Transgenic Zebrafish[J].Environmental Monitoring and Forewarning,2020,12(5):102-107 |
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| 摘要: |
| 为探究2,2’,4,4’-四溴联苯醚（BDE-47）对斑马鱼的早期神经毒性作用，采用中枢神经特异性标记的转基因斑马鱼Tg(elavl3:EGFP)作为模式生物，研究了BDE-47短期暴露对斑马鱼幼鱼的运动行为轨迹、中枢神经发育和神经发育关键基因表达的影响。结果表明，BDE-47暴露6 d后，5和10μmol/L暴露组斑马鱼幼鱼运动行为和神经发育关键基因（elavl3和mbp）的表达受到显著抑制，同时斑马鱼幼鱼的神经细胞的发育受到显著抑制。结果提示，神经系统特异性标记的转基因斑马鱼可作为一种筛选神经毒性化合物的理想动物模型，BDE-47对斑马鱼具有早期神经发育毒性。 |
| 关键词: 2,2’,4,4’-四溴联苯醚 转基因斑马鱼 神经毒性 |
| DOI： |
| 分类号:X835 |
| 文献标识码:B |
| 基金项目:江苏环保科研课题基金资助项目（2018001）；中央级公益性科研院所基本科研业务费专项基金资助项目（GYZX200102） |
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| Detecting the Neurotoxicity Effects of 2,2’,4,4’Tetrabromodiphenyl Ether Using Transgenic Zebrafish |
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GU Jie, GUO Min, JI Gui-xiang, SHI Li-li
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Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing, Jiangsu 210042, China
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| Abstract: |
| In order to determine the early neurotoxicity effects of 2,2’,4,4’tetrabromodiphenyl ether (BDE-47) on zebrafish, the effects of short term BDE-47 exposure on motor behavior trajectory, central nervous development and nervous system expression of key genes in zebrafish larva were studied by using central nervous system specific transgenic zebrafish Tg (elavl3:EGFP) as a model organism. The results showed that after 6 days of BDE-47 exposure, the expressions of key genes (elavl3 and mbp) in motor behavior and neurodevelopment of zebrafish larva exposed to 5 and 10 μmol/L were significantly inhibited. At the same time, the development of nerve cells of zebrafish larva was significantly inhibited. The results suggest that nervous system specific transgenic zebrafish can be used as an ideal animal model for screening neurotoxic compounds, and BDE-47 has early neurodevelopmental toxicity to zebrafish. |
| Key words: BDE-47 Transgenic zebrafish Neurotoxicity |
