引用本文:刘鹏,车子凡,张徐祥.水环境中病毒检测技术研究进展[J].环境监控与预警,2021,13(3):1-7
LIU Peng, CHE Zi-fan, ZHANG Xu-xiang.Progress in Detection Technologies of Viruses in Water Environment[J].Environmental Monitoring and Forewarning,2021,13(3):1-7
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水环境中病毒检测技术研究进展
刘鹏,车子凡,张徐祥
作者单位
刘鹏,车子凡,张徐祥* 污染控制与资源化研究国家重点实验室南京大学环境学院江苏 南京 210023 
摘要:
综述了水环境病毒检测技术的研究进展。介绍了过滤法、吸附法、离心法、混凝法等水环境病毒富集方法的原理和优缺点;总结了基于聚合酶链式反应(PCR),包括巢式PCR、多重PCR、实时荧光定量PCR、细胞联合培养PCR和数字PCR,以及高通量测序等分子生物学技术在水环境病毒检测中的应用;指出了水环境中病毒检测技术当前存在的问题和未来发展方向,为水环境中病毒的检测与管控提供技术支撑。
关键词:  水环境  病毒检测  富集  分子生物学技术
DOI:
分类号:X831;R123.1
文献标识码:A
基金项目:国家自然科学基金资助项目(52025102);江苏省研究生科研与实践创新计划基金资助项目(KYCX18_0034);南京大学优秀博士研究生创新能力提升计划B资助项目(202002B079)
Progress in Detection Technologies of Viruses in Water Environment
LIU Peng, CHE Zi-fan, ZHANG Xu-xiang
Abstract:
This study briefly reviewed detection technologies of viruses in water environment. In this study, we introduced several methods for the enrichment of viruses in water environment and analyzed their principles, advantages and disadvantages. Besides, we also summarized the application of molecular biotechnologies in viruses detection in water environment, such technologies includes PCR-based molecular methods like nested PCR, multiplex PCR, quantitative real-time PCR, integrated cell culture PCR, digital PCR and high-throughput sequencing. Furthermore, the review provides references for future research on detection technologies of viruses in water environment, which can contribute positively to the monitoring and control of viruses in water environment.
Key words:  Water environment  Viruses detection  Enrichment  Molecular biotechnology